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KMID : 0893420130140040405
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Journal of Veterinary Science
2013 Volume.14 No. 4 p.405 ~ p.412
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Inhibitory effects of osteoprotegerin on osteoclast formation and function under serum-free conditions
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Fu Ying Xiao
Gu Jian Hong
Zhang Yi Ran
Tong Xi Shuai
Zhao Hong Yan
Yuan Yan
Liu Xue Zhong
Bian Jian Chun
Liu Zong Ping
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Abstract
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The purpose of this study was to determine whether osteoprotegerin (OPG) could affect osteoclat differentiation and activation under serum-free conditions. Both duck embryo bone marrow cells and RAW264.7 cells were incubated with macrophage colony stimulatory factor (M-CSF) and receptor activator for nuclear factor κB ligand (RANKL) in serum-free medium to promote osteoclastogenesis. During cultivation, 0, 10, 20, 50, and 100 ng/mL OPG were added to various groups of cells. Osteoclast differentiation and activation were monitored via tartrate-resistant acid phosphatase (TRAP) staining, filamentous-actin rings analysis, and a bone resorption assay. Furthermore, the expression osteoclast-related genes, such as TRAP and receptor activator for nuclear factor κB (RANK), that was influenced by OPG in RAW264.7 cells was examined using real-time polymerase chain reaction. In summary, findings from the present study suggested that M-CSF with RANKL can promote osteoclast differentiation and activation, and enhance the expression of TRAP and RANK mRNA in osteoclasts. In contrast, OPG inhibited these activities under serum-free conditions.
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KEYWORD
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activation, differentiation, osteoclast, osteoprotegerin, serum-free
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