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KMID : 0893420130140040491
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Journal of Veterinary Science
2013 Volume.14 No. 4 p.491 ~ p.494
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Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
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Garcia Alfredo
Martinez Remigio
Benitez Medina Jose Manuel
Risco David
Garcia Waldo Luis
Rey Joaquin
Alonso Juan Manuel
Mendoza Javier Hermoso
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Abstract
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Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
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KEYWORD
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clinical samples, Dermatophilus congolensis, dermatophilosis, diagnosis, real-time PCR
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