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KMID : 0893420140150010027
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Journal of Veterinary Science
2014 Volume.15 No. 1 p.27 ~ p.33
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Cell proliferation and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice are increased after rosiglitazone treatment
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Yoo Dae-Young
Kim Woo-Suk
Kim Dae-Won
Nam Sung-Min
Jung Hyo-Young
Kim Jong-Whi
Lee Choong-Hyun
Choi Jung-Hoon
Won Moo-Ho
Yoon Yeo-Sung
Hwan In-Koo
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Abstract
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In this study, we determined how rosiglitazone (RSG) differentially affected hippocampal neurogenesis in mice fed a low-fat diet (LFD) or high-fat diet (HFD; 60% fat). LFD and HFD were given to the mice for 8 weeks. Four weeks after initiating the LFD and HFD feeding, vehicle or RSG was administered orally once a day to both groups of mice. We measured cell proliferation and neuroblast differentiation in the subgranular zone of the dentate gyrus using Ki67 and doublecortin (DCX), respectively, as markers. In addition, we monitored the effects of RSG on the levels of DCX and brain-derived neurotrophic factor (BDNF) in hippocampal homogenates. At 8 weeks after the LFD feeding, the numbers of Ki67- and DCX-positive cells as well as hippocampal levels of DCX and BDNF were significantly decreased in the RSG-treated group compared to the vehicle-treated animals. In contrast, the numbers of Ki67- and DCX-positive cells along with hippocampal levels of DCX and BDNF in the HFD fed mice were significantly increased in the RSG-treated mice compared to the vehicle-treated group. Our data demonstrate that RSG can modulate the levels of BDNF, which could play a pivotal role in cell proliferation and neuroblast differentiation in the hippocampal dentate gyrus.
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KEYWORD
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brain-derived neurotrophic factor, dentate gyrus, high-fat diet, rosiglitazone
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