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KMID : 0893420140150010163
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Journal of Veterinary Science
2014 Volume.15 No. 1 p.163 ~ p.166
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Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus)
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Park Seong-Bin
Kwon Kyoung
Cha In-Seok
Jang Ho-Bin
Nho Seong-Won
Fagutao Fernand F.
Kim Young-Kyu
Yu Jong-Earn
Jung Tae-Sung
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Abstract
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A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea.
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KEYWORD
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Edwardsiella tarda, multiplex PCR, olive flounder, Streptococcus iniae, Streptococcus parauberis
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