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KMID : 0893420140150030389
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Journal of Veterinary Science
2014 Volume.15 No. 3 p.389 ~ p.398
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Molecular characterization of duck enteritis virus CHv strain UL49.5 protein and its colocalization with glycoprotein M
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Lin Meng
Jia Renyong
Wang Mingshu
Gao Xinghong
Zhu Dekang
Chen Shun
Liu Mafeng
Yin Zhongqiong
Wang Yin
Chen Xiaoyue
Cheng Anchun
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Abstract
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The UL49.5 gene of most herpesviruses is conserved and encodes glycoprotein N. However, the UL49.5 protein of duck enteritis virus (DEV) (pUL49.5) has not been reported. In the current study, the DEV pUL49.5 gene was first subjected to molecular characterization. To verify the predicted intracellular localization of gene expression, the recombinant plasmid pEGFP-C1/pUL49.5 was constructed and used to transfect duck embryo fibroblasts. Next, the recombinant plasmid pDsRed1-N1/glycoprotein M (gM) was produced and used for co-transfection with the pEGFP-C1/pUL49.5 plasmid to determine whether DEV pUL49.5 and gM (a conserved protein in herpesviruses) colocalize. DEV pUL49.5 was thought to be an envelope glycoprotein with a signal peptide and two transmembrane domains. This protein was also predicted to localize in the cytoplasm and endoplasmic reticulum with a probability of 66.7%. Images taken by a fluorescence microscope at different time points revealed that the DEV pUL49.5 and gM proteins were both expressed in the cytoplasm. Overlap of the two different fluorescence signals appeared 12 h after transfection and continued to persist until the end of the experiment. These data indicate a possible interaction between DEV pUL49.5 and gM.
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KEYWORD
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colocalization, duck enteritis virus, intracellular localization, molecular characterization, UL49.5 protein
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