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KMID : 0893420140150030423
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Journal of Veterinary Science
2014 Volume.15 No. 3 p.423 ~ p.426
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A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C
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Ding Yao zhong
Zhou Jian hua
Ma Li na
Qi Yan ni
Wei Gang
Zhang Jie
Zhang Yong guang
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Abstract
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A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription-PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.
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KEYWORD
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foot-and-mouth disease, foot-and-mouth disease virus serotype C, reverse transcription loop-mediated isothermal amplification, reverse transcription-PCR, sensitivity
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