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KMID : 0893420140150040475
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Journal of Veterinary Science
2014 Volume.15 No. 4 p.475 ~ p.483
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Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage
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Wu Li ming
Guo Rui
Hui Lin
Ye Yong gang
Xiang Jing mei
Wan Chun yun
Zou Miao
Ma Rui
Sun Xiao zhuan
Yang Shi jin
Guo Ding zong
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Abstract
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Chronic enteritis can produce an excess of reactive oxygen species resulting in cellular damage. Stanniocalcin-1(STC-1) reportedly possesses anti-oxidative activity, the aim of this study was to define more clearly the direct contribution of STC-1 to anti-oxidative stress in cattle. In this study, primary intestinal epithelial cells (IECs) were exposed to hydrogen peroxide (H2O2) for different time intervals to mimic chronic enteritis-induced cellular damage. Prior to treatment with 200 μM H2O2, the cells were transfected with a recombinant plasmid for 48 h to over-express STC-1. Acridine orange/ethidium bromide (AO/EB) double staining and trypan blue exclusion assays were then performed to measure cell viability and apoptosis of the cells, respectively. The expression of STC-1 and apoptosis-related proteins in the cells was monitored by real-time PCR and Western blotting. The results indicated that both STC-1 mRNA and protein expression levels positively correlated with the duration of H2O2 treatment. H2O2 damaged the bovine IECs in a time-dependent manner, and this effect was attenuated by STC-1 over-expression. Furthermore, over-expression of STC-1 up-regulated Bcl-2 protein expression and slightly down-regulated caspase-3 production in the damaged cells. Findings from this study suggested that STC-1 plays a protective role in intestinal cells through an antioxidant mechanism.
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KEYWORD
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Bcl-2, chronic enteritis, oxidative damage, stanniocalcin-1
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