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KMID : 0893420150160010001
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Journal of Veterinary Science
2015 Volume.16 No. 1 p.1 ~ p.9
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β-irradiation (166Ho patch)-induced skin injury in mini-pigs: effects on NF-κB and COX-2 expression in the skin
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Kim Joong-Sun
Rhim Kyung-Jin
Jang Won-Seok
Lee Sun-Joo
Son Yeong-Hoon
Lee Seung-Sook
Park Sun-Hoo
Lim Sang-Moo
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Abstract
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In the present study, the detrimental effect of β-emission on pig skin was evaluated. Skin injury was modeled in mini-pigs by exposing the animals to 50 and 100 Gy of β-emission delivered by 166Ho patches. Clinicopathological and immunohistochemical changes in exposed skin were monitored for 18 weeks after β-irradiation. Radiation induced desquamation at 2~4 weeks and gradual repair of this damage was evident 6 weeks after irradiation. Changes in basal cell density and skin depth corresponded to clinically relevant changes. Skin thickness began to decrease 1 week after irradiation, and the skin was thinnest 4 weeks after irradiation. Skin thickness increased transiently during recovery from irradiation-induced skin injury, which was evident 6~8 weeks after irradiation. Epidermal expression of nuclear factor-kappa B (NF-κB) differed significantly between the untreated and irradiated areas. One week after irradiation, cyclooxygenase-2 (COX-2) expression was mostly limited to the basal cell layer and scattered among these cells. High levels of COX-2 expression were detected throughout the full depth of the skin 4 weeks after irradiation. These findings suggest that NF-κB and COX-2 play roles in epidermal cell regeneration following β-irradiation of mini-pig skin.
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KEYWORD
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β-ray, irradiation, pig, skin
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