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KMID : 0893420150160010017
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Journal of Veterinary Science
2015 Volume.16 No. 1 p.17 ~ p.23
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Protective effect of butylated hydroxylanisole against hydrogen peroxide-induced apoptosis in primary cultured mouse hepatocytes
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Hwang Geun-Hye
Jeon Yu-Jin
Han Ho-Jae
Park Soo-Hyun
Baek Kyoung-Min
Chang Woo-Chul
Kim Joong-Sun
Kim Lark-Kyun
Lee You-Mie
Lee Sang-Kyu
Bae Jong-Sup
Jee Jun-Goo
Lee Min-Young
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Abstract
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Butylated hydroxyanisole (BHA) is a synthetic phenolic compound consisting of a mixture of two isomeric organic compounds: 2-tert-butyl-4-hydroxyanisole and 3-tert-butyl-4-hydroxyanisole. We examined the effect of BHA against hydrogen peroxide (H2O2)-induced apoptosis in primary cultured mouse hepatocytes. Cell viability was significantly decreased by H2O2 in a dose-dependent manner. Additionally, H2O2 treatment increased Bax, decreased Bcl-2, and promoted PARP-1 cleavage in a dose-dependent manner. Pretreatment with BHA before exposure to H2O2 significantly attenuated the H2O2-induced decrease of cell viability. H2O2 exposure resulted in an increase of intracellular reactive oxygen species (ROS) generation that was significantly inhibited by pretreatment with BHA or N-acetyl-cysteine (NAC, an ROS scavenger). H2O2-induced decrease of cell viability was also attenuated by pretreatment with BHA and NAC. Furthermore, H2O2-induced increase of Bax, decrease of Bcl-2, and PARP-1 cleavage was also inhibited by BHA. Taken together, results of this investigation demonstrated that BHA protects primary cultured mouse hepatocytes against H2O2-induced apoptosis by inhibiting ROS generation.
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KEYWORD
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apoptosis, butylated hydroxyanisole, primary mouse hepatocytes, reactive oxygen species
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