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KMID : 0893420150160010031
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Journal of Veterinary Science
2015 Volume.16 No. 1 p.31 ~ p.35
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Performance of the SD Bioline TB Ag MPT64 Rapid test for quick confirmation of Mycobacterium bovis isolates from animals
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Byeon Hyeon-Seop
Ji Mi-Jung
Kang Shin-Seok
Kim Sang-Woo
Kim Seung-Cheol
Park Song-Yong
Kim Gee-Hyuk
Kim Ji-Ro
Cho Jang-Eun
Ku Bok-Kyung
Kim Jae-Myung
Jeon Bo-young
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Abstract
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Mycobacterium (M.) bovis, a bacterium in the M. tuberculosis complex, is a causative agent of bovine tuberculosis, a contagious disease of animals. Mycobacterial culture is the gold standard for diagnosing bovine tuberculosis, but this technique is laborious and time-consuming. In the present study, performance of the SD Bioline TB Ag MPT4 Rapid test, an immunochromatographic assay, was evaluated using reference bacterial strains and M. bovis field isolates collected from animals. The SD MPT64 Rapid test produced positive results for 95.5% (63/66) of the M. bovis isolates from cattle and 97.9% (46/47) of the isolates from deer. Additionally, the test had a sensitivity of 96.5% (95% CI, 91.2-99.0), specificity of 100% (95% CI, 96.7-100.0), positive predictive value of 100% (95% CI, 96.7-100.0), and negative predictive value of 92.9% (95% CI, 82.7-98.0) for M. bovis isolates. In conclusion, the SD MPT64 Rapid test is simple to use and may be useful for quickly confirming the presence of M. bovis in animals.
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KEYWORD
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animals, Mycobacterium bovis, SD MPT64 Rapid test
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