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KMID : 0893420150160020203
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Journal of Veterinary Science
2015 Volume.16 No. 2 p.203 ~ p.211
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A study of experimental autoimmune encephalomyelitis in dogs as a disease model for canine necrotizing encephalitis
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Moon Jong-Hyun
Jung Hae-Won
Lee Hee-Chun
Jeon Joon-Hyeok
Kim Na-Hyun
Sur Jung-Hyang
Ha Jeong-Im
Jung Dong-In
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Abstract
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In the present study, the use of dogs with experimental autoimmune encephalomyelitis (EAE) as a disease model for necrotizing encephalitis (NE) was assessed. Twelve healthy dogs were included in this study. Canine forebrain tissues (8 g), including white and grey matter, were homogenized with 4 mL of phosphate-buffered saline for 5 min in an ice bath. The suspension was emulsified with the same volume of Freund's complete adjuvant containing 1 mg/mL of killed Mycobacterium tuberculosis H37Ra. Under sedation, each dog was injected subcutaneously with canine brain homogenate at four sites: two in the inguinal and two in the axillary regions. A second injection (booster) was administered to all the dogs using the same procedure 7 days after the first injection. Clinical assessment, magnetic resonance imaging, cerebrospinal fluid analyses, necropsies, and histopathological and immunohistochemical examinations were performed for the dogs with EAE. Out of the 12 animals, seven (58%) developed clinically manifest EAE at various times after immunization. Characteristics of canine EAE models were very similar to canine NE, suggesting that canine EAE can be a disease model for NE in dogs.
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KEYWORD
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dog, experimental autoimmune encephalomyelitis, multiple sclerosis, necrotizing encephalitis, necrotizing meningoencephalitis
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