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KMID : 0893420150160020233
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Journal of Veterinary Science
2015 Volume.16 No. 2 p.233 ~ p.235
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In vitro development of canine somatic cell nuclear transfer embryos in different culture media
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Kim Dong-Hoon
No Jin-Gu
Choi Mi-Kyung
Yeom Dong-Hyeon
Kim Dong-Kyo
Yang Byoung-Chul
Yoo Jae-Gyu
Kim Min-Kyu
Kim Hong-Tea
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Abstract
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The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%) or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos.
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KEYWORD
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canine, G1/G2 media, in vitro culture, somatic cell nuclear transfer embryo
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