|
KMID : 0893420150160040413
|
|
Journal of Veterinary Science
2015 Volume.16 No. 4 p.413 ~ p.421
|
|
|
Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy
|
|
Meyerholz Marie M
Mense Kirsten
Lietzau Michael
Kassens Ana
Linden Matthias
Knaack Hendrike
Wirthgen Elisa
Hoeflich Andreas
Raliou Mariam
Richard Christophe
Sandra Olivier
Schuberth Hans Joachim
Hoedemaker Martina
Schmicke Marion
|
|
|
Abstract
|
|
|
|
This study was conducted to determine if the main components of the somatotropic axis change during the early phase of pregnancy in the maternal blood system and whether differences exist on day 18 after pregnancy recognition by the maternal organism. Blood samples of pregnant heifers (Holstein Friesian; n = 10 after embryo transfer) were obtained on the day of ovulation (day 0), as well as on days 7, 14, 16 and 18 and during pregnant, non-pregnant and negative control cycles. The oncentrations of progesterone (P4), oestrogen, growth hormone (GH), insulin-like growth factor-1 and -2 (IGF1, -2) and IGF-binding protein-2, -3 and -4 (IGFBP2, -3, -4) were measured. The mRNA expressions of growth hormone receptor 1A, IGF1, IGF2, IGFBP2, IGFBP3 and IGFBP4 were detected using RT-qPCR in liver biopsy specimens (day 18). In all groups, total serum IGF1 decreased from day 0 to 16. Notably, IGFBP4 maternal blood concentrations were lower during pregnancy than during non-pregnant cycles and synchronized control cycles. It can be speculated that the lower IGFBP4 in maternal blood may result in an increase of free IGF1 for local action. Further studies regarding IGFBP4 concentration and healthy early pregnancy are warranted.
|
|
|
KEYWORD
|
|
|
dairy cattle, insulin-like growth factor-1, insulin-like growth factor-2, insulin-like growth factor-binding protein-2, pregnancy
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
   
|
|