KMID : 0893420150160040483
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Journal of Veterinary Science 2015 Volume.16 No. 4 p.483 ~ p.489
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Profiling of antimicrobial resistance and plasmid replicon types in ¥â-lactamase producing Escherichia coli isolated from Korean beef cattle
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Shin Seung-Won
Jung Myung-Hwan Shin Min-Kyung Yoo Han-Sang
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Abstract
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In this study, 78 isolates of Escherichia coli isolated from Korean beef cattle farms were investigated for the production of extended-spectrum ¥â-lactamase (ESBL) and/or AmpC ¥â-lactamase. In the disc diffusion test with ampicillin, amoxicillin, cephalothin, ceftiofur, cefotaxime, ceftazidime, and cefoxitin, 38.5% of the isolates showed resistance to all of ampicillin, amoxicillin, and cephalothin. The double disc synergy method revealed that none of the isolates produced ESBL or AmpC ¥â-lactamases. DNA sequencing showed that all isolates encoded genes for TEM-1-type ¥â-lactamase. Moreover, 78.2% of the isolates transferred the TEM-1-type ¥â-lactamase gene via conjugation. In plasmid replicon typing of all donors, IncFIB and IncFIA were identified in 71.4% and 41.0% of plasmids, respectively. In transconjugants, IncFIB and IncFIA were the most frequent types detected (61.5% and 41.0%, respectively). Overall, the present study indicates that selection pressures of antimicrobials on ¥â-lactamases in beef cattle may be low relative to other livestock animals in Korea. Moreover, to reduce selection pressure and dissemination of ¥â-lactamase, the long-term surveillance of antimicrobial use in domestic beef cattle should be established.
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KEYWORD
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¥â-lactamase, antimicrobial resistance, Escherichia coli, plasmid replicon typing
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