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KMID : 0893420160170030289
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Journal of Veterinary Science
2016 Volume.17 No. 3 p.289 ~ p.297
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Immunologic properties of differentiated and undifferentiated mesenchymal stem cells derived from umbilical cord blood
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Lee Hyo-Jong
Kang Kyung-Sun
Kang Sun-Young
Kim Hyung-Sik
Park Se-Jin
Lee Seung-Yong
Kim Kwang-Dong
Lee Hee-Chun
Park Ji-Kwon
Paik Won-Young
Lee Lyon
Yeon Seong-Chan
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Abstract
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The expression of immunogenic markers after differentiation of umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and requires extensive in vitro and in vivo testing for clinical application. The expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested by Fluorescence-activated cell sorting analysis and immunocytochemical staining. The undifferentiated MSC were moderately positive for HLA-ABC, but almost completely negative for HLA-DR. The MSC differentiated to chondrocytes expressed neither HLA-ABC nor HLA-DR. The proliferation of MSC was not significantly affected by the allogeneic lymphocytes stimulated with concanavalin A. The responder lymphocytes showed no significant decrease in proliferation in the presence of the MSC, but the apoptosis rate of the lymphocytes was increased in the presence of MSC. Taken together, these findings indicate that UCB-derived MSC differentiated to chondrocytes expressed less HLA class I and no class II antigens. The MSC showed an immunomodulatory effect on the proliferation and apoptosis of allogeneic lymphocytes. These data suggest that the differentiated and undifferentiated allogeneic MSC derived from umbilical cord blood can be a useful candidate for allogeneic cell therapy and transplantation without a major risk of rejection.
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KEYWORD
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apoptosis, human leukocyte antigen, human umbilical cord blood, immunophenotypes, mesenchymal stem cells
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