|
KMID : 0893420160170030299
|
|
Journal of Veterinary Science
2016 Volume.17 No. 3 p.299 ~ p.306
|
|
|
Molecular characterization of highly pathogenic avian influenza H5N8 viruses isolated from Baikal teals found dead during a 2014 outbreak in Korea
|
|
Kim Seol-Hee
Hur Moon-Suk
Suh Jae-Hwa
Woo Chan-Jin
Wang Seung-Jun
Park Eung-Roh
Hwang Jong-Kyung
An In-Jung
Jo Seong-Deok
Shin Jeong-Hwa
Yu Seung-Do
Choi Kyung-Hee
Lee Dong-Hun
Song Chang-Seon
|
|
|
Abstract
|
|
|
|
Nineteen highly pathogenic avian influenza (HPAI) H5N8 viruses were isolated from wild birds in the Donglim reservoir in Gochang, Jeonbuk province, Korea, which was first reported to be an outbreak site on January 17, 2014. Most genes from the nineteen viruses shared high nucleotide sequence identities (i.e., 99.7% to 100%). Phylogenetic analysis showed that these viruses were reassortants of the HPAI H5 subtype and the H4N2 strain and that their hemagglutinin clade was 2.3.4.4, which originated from Eastern China. The hemagglutinin protein contained Q222 and G224 at the receptor-binding site. Although the neuraminidase protein contained I314V and the matrix 2 protein contained an S31N substitution, other mutations resulting in oseltamivir and amantadine resistance were not detected. No substitutions associated with increased virulence and enhanced transmission in mammals were detected in the polymerase basic protein 2 (627E and 701D). Non-structural-1 was 237 amino acids long and had an ESEV motif with additional RGNKMAD amino acids in the C terminal region. These viruses caused deaths in the Baikal teal, which was unusual, and outbreaks occurred at the same time in both poultry and wild birds. These data are helpful for epidemiological understanding of HPAI and the design of prevention strategies.
|
|
|
KEYWORD
|
|
|
H5N8, avian influenza, phylogenetic analysis, surveillance, wild bird
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
   
|