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KMID : 0893420170180010101
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Journal of Veterinary Science
2017 Volume.18 No. 1 p.101 ~ p.104
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Stage-specific embryonic antigen: determining expression in canine glioblastoma, melanoma, and mammary cancer cells
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Lin Weiming
Modiano Jaime F
Ito Daisuke
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Abstract
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The expression of stage-specific embryonic antigens (SSEAs) was determined in several types of canine cancer cells. Flow cytometry showed SSEA-1 expression in glioblastoma, melanoma, and mammary cancer cells, although none expressed SSEA-3 or SSEA-4. Expression of SSEA-1 was not detected in lymphoma, osteosarcoma, or hemangiosarcoma cell lines. Relatively stable SSEA-1 expression was observed between 24 and 72 h of culture. After 8 days in culture, sorted SSEA-1? and SSEA-1+ cells re-established SSEA-1 expression to levels comparable to those observed in unsorted cells. Our results document, for the first time, the expression of SSEA-1 in several canine cancer cell lines.
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KEYWORD
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canine cancer, flow cytometry, magnetic-activated cell sorting, stage-specific embryonic antigens
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