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KMID : 0893420170180020159
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Journal of Veterinary Science
2017 Volume.18 No. 2 p.159 ~ p.167
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Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice
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Liu Yu Ying
Yang Wen Tao
Shi Shao Hua
Li Ya Jie
Zhao Liang
Shi Chun Wei
Zhou Fang Yu
Jiang Yan Long
Hu Jing Tao
Gu Wei
Yang Gui Lian
Wang Chun Feng
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Abstract
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Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study, Lactobacillus (L.) plantarum NC8 was selected as a vector to express the VP2 gene of GPV, and recombinant L. plantarum pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 × 109 colony-forming unit/200 μL dose of the recombinant L. plantarum strain, and the ratios and numbers of CD11c+, CD3+CD4+, CD3+CD8+, and interferon gamma- and tumor necrosis factor alpha-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in the control groups. In addition, we assessed the capacity of L. plantarum SIP409-VP2/NC8 to induce secretory IgA production. We conclude that administered pSIP409-VP2/NC8 leads to relatively extensive cellular responses. This study provides information on GPV infection and offers a clear framework of options available for GPV control strategies.
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KEYWORD
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Lactobacillus plantarum, VP2 gene, goose parvovirus, immunization
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