KMID : 0893420170180040547
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Journal of Veterinary Science 2017 Volume.18 No. 4 p.547 ~ p.549
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Surveyor assay to diagnose persistent Mullerian duct syndrome in Miniature Schnauzers
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Kim Young-June
Kwon Hyuk-Jin Byun Hyuk-Soo Yeom Don-Guk Choi Jea-Hong Kim Joong-Hyun Shim Ho-Sup
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Abstract
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Persistent Mullerian duct syndrome (PMDS) is a pseudohermaphroditism in males characterized by the presence of Mullerian duct derivatives. As PMDS dogs often lack clinical symptoms, a molecular diagnosis is essential to identify the syndrome in these animals. In this study, a new molecular method using DNA mismatch-specific Surveyor nuclease was developed. The Surveyor nuclease assay identified the AMHR2 mutation that produced PMDS in a Miniature Schnauzer as accurately as that obtained by using the conventional method based on restriction digestion. As an alternative to the current molecular diagnostic method, the new method may result in increased accuracy when detecting PMDS.
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KEYWORD
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Miniature Schnauzer, persistent Mullerian duct syndrome, surveyor nuclease assay
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