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KMID : 0893420180190010035
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Journal of Veterinary Science
2018 Volume.19 No. 1 p.35 ~ p.43
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Analysis of structure-function relationship in porcine rotavirus A enterotoxin gene
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Saurabh Sharad
Sircar Shubhankar
Kattoor Jobin J.
Ghosh Souvik
Kobayashi Nobumichi
Banyai Krisztian
VinodhKumar Obli R.
De Ujjwal K.
Sahoo Nihar R.
Dhama Kuldeep
Malik Yashpal S.
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Abstract
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Rotavirus (RV)-infected piglets are presumed to be latent sources of heterologous RV infection in humans and other animals. In RVs, non-structural protein 4 (NSP4) is the major virulence factor with pleiotropic properties. In this study, we analyzed the nsp4 gene from porcine RVs isolated from diarrheic and non-diarrheic cases at different levels of protein folding to explore correlations to diarrhea-inducing capabilities and evolution of nsp4 in the porcine population. Full-length nsp4 genes were amplified, cloned, sequenced, and then analyzed for antigenic epitopes, RotaC classification, homology, genetic relationship, modeling of NSP4 protein, and prediction of post-translational modification. RV presence was observed in both diarrheic and non-diarrheic piglets. All nsp4 genes possessed the E1 genotype. Comparison of primary, secondary, and tertiary structure and the prediction of post-translational modifications of NSP4 from diarrheic and non-diarrheic piglets revealed no apparent differences. Sequence analysis indicated that nsp4 genes have a multi-phyletic evolutionary origin and exhibit species independent genetic diversity. The results emphasize the evolution of the E9 nsp4 genotype from the E1 genotype and suggest that the diarrhea-inducing capability of porcine RVs may not be exclusively linked to its enterotoxin gene.
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KEYWORD
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enterotoxins, nsp4 gene evolution, porcine, rotavirus, viral nonstructural proteins
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