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KMID : 0893420180190050667
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Journal of Veterinary Science
2018 Volume.19 No. 5 p.667 ~ p.675
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Effect of superoxide dismutase, catalase, and glutathione peroxidase supplementation in the extender on chilled semen of fertile and hypofertile dogs
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Del Prete Chiara
Ciani Francesca
Tafuri Simona
Pasolini Maria Pia
Valle Giovanni Della
Palumbo Veronica
Abbondante Lucia
Calamo Antonio
Barbato Vincenza
Gualtieri Roberto
Talevi Riccardo
Cocchia Natascia
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Abstract
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This study investigated the correlation between oxidative stress status and key canine sperm parameters and the effect of addition of a superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) combination in egg yolk tris-citrate glucose (EYT-G) extender on semen during 10 days of storage at 4oC. Ten Boxer dogs were divided into two groups, fertile (F) and hypofertile (H), depending on pregnancy and live birth rate status in the previous year. Semen evaluation was performed on the day of collection (D0) and after 5 (D5) and 10 (D10) days of cooled storage. Sperm motility, kinetic parameters, and DNA integrity were assessed. A correlation between oxidative status and key semen parameters in both F and H groups was observed. Total and progressive motilities were significantly higher in the treated (SOD, CAT, and GPx addition) versus control groups at D10 in both F and H groups, and at D5 in the H group. DNA integrity was significantly higher in both treated groups (H and F) at D5 and D10. In conclusion, the addition of SOD, CAT, and GPx in the extender allows preservation of semen quality for up to 10 days of storage at 4oC in both fertile and hypofertile dogs.
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KEYWORD
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antioxidants, canine sperm, fertility, oxidative stress, semen preservation
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