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KMID : 0893420190200030028
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Journal of Veterinary Science
2019 Volume.20 No. 3 p.28 ~ p.28
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Histopathological and microbiological findings in buffalo chronic mastitis: evidence of tertiary lymphoid structures
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Restucci Brunella
Dipineto Ludovico
Martano Manuela
Balestrieri Anna
Ciccarelli Davide
Russo Tamara Pasqualina
Varriale Lorena
Maiolino Paola
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Abstract
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Buffalo mastitis is an important economic problem in southern Italy, causing qualitative/quantitative alterations in milk and resulting in economic losses due to the sub-clinical course and chronic evolution. We investigated 50 udders of slaughtered buffaloes and subjected them to effectual microbiological screening to evaluate macro and microscopic mammary gland changes, immune-characterize the cell infiltrates, and compare the degree of tissue inflammation with somatic cell counts. Numerous Gram-positive and Gram-negative bacteria were isolated from all samples, majority of which were environmental mastitis pathogens. Histological features referable to chronic mastitis were observed in 92% udders. Lymphocytes, plasma cells and macrophages were found to evolve into aggregates in 48% udders, which often organized to form tertiary lymphoid structures (TLSs). A predominance of interstitial CD8+ over CD4+ lymphocytes and, in TLSs, scattered CD8+ lymphocytes in the mantle cells and CD79+ lymphocytes in germinal centers, were evidenced. Environmental pathogens are known to persist and cause chronic inflammatory changes in buffaloes, where CD8+ lymphocytes play an important role by controlling the local immune response. Moreover, the TLSs evidenced here for the first time in buffalo mastitis, could play a role in maintaining immune responses against persistent antigens, thereby contributing in determining the chronic course of mastitis.
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KEYWORD
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Chronic mastitis, buffalo, histopathology, microbiology, tertiary lymphoid structures
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