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KMID : 0893420200210060081
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Journal of Veterinary Science
2020 Volume.21 No. 6 p.81 ~ p.81
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Identification of urinary microRNA biomarkers for in vivo gentamicin-induced nephrotoxicity models
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Jeon Byung-Suk
Lee Soo-Ho
Hwang So-Ryeon
Yi Hee
Bang Ji-Hyun
Tham Nga Thi Thu
Lee Hyun-Kyoung
Woo Gye-Hyeong
Kang Hwan-Goo
Ku Hyun-Ok
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Abstract
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Background: Although previous in vivo studies explored urinary microRNA (miRNA), there is no agreement on nephrotoxicity-specific miRNA biomarkers.
Objectives: In this study, we assessed whether urinary miRNAs could be employed as biomarkers for nephrotoxicity.
Methods: For this, literature-based candidate miRNAs were identified by reviewing the previous studies. Female Sprague-Dawley rats received subcutaneous injections of a single dose or repeated doses (3 consecutive days) of gentamicin (GEN; 137 or 412 mg/kg). The expression of miRNAs was analyzed by real-time reverse transcription-polymerase chain reaction in 16 h pooled urine from GEN-treated rats.
Results: GEN-induced acute kidney injury was confirmed by the presence of tubular necrosis. We identified let-7g-5p, miR-21-3p, 26b-3p, 192-5p, and 378a-3p significantly upregulated in the urine of GEN-treated rats with the appearance of the necrosis in proximal tubules. Specifically, miR-26-3p, 192-5p, and 378a-3p with highly expressed levels in urine of rats with GEN-induced acute tubular injury were considered to have sensitivities comparable to clinical biomarkers, such as blood urea nitrogen, serum creatinine, and urinary kidney injury molecule protein.
Conclusions: These results indicated the potential involvement of urinary miRNAs in chemical-induced nephrotoxicity, suggesting that certain miRNAs could serve as biomarkers for acute nephrotoxicity.
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KEYWORD
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Acute kidney injury, biomarker, gentamicin, microRNAs, nephrotoxicity
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