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KMID : 0893420200210060085
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Journal of Veterinary Science
2020 Volume.21 No. 6 p.85 ~ p.85
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Generation of a cold-adapted PRRSV with a nucleotide substitution in the ORF5 and numerous mutations in the hypervariable region of NSP2
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Do Van Tan
Dao Hoai Thu
Hahn Tae-Wook
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Abstract
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A cold-adapted porcine reproductive and respiratory syndrome virus (CA-VR2332) was generated from the modified live virus strain VR2332. CA-VR2332 showed impaired growth when cultured at 37°C with numerous mutations (S731F,E819D,G975E, and D1014N) in the hypervariable region of the NSP2, in which the mutation S731F might play a vital role in viral replication at 30°C. Conserved amino acid sequences of the GP5 protein suggests that CA-VR2332 is a promising candidate for producing an effective vaccine against PRRSV infection. Further studies on replication and immunogenicity in vivo are required to evaluate the properties of CA-VR2332.
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KEYWORD
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Porcine reproductive and respiratory syndrome, cold-adapted, GP5, hypervariable region, NSP2
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