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KMID : 0893420210220010008
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Journal of Veterinary Science
2021 Volume.22 No. 1 p.8 ~ p.8
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Expression and evaluation of porcine circovirus type 2 capsid protein mediated by recombinant adeno-associated virus 8
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Li Shuang
Wang Bo
Jiang Shun
Lan Xiaohui
Qiao Yongbo
Nie Jiaojiao
Yin Yuhe
Shi Yuhua
Kong Wei
Shan Yaming
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Abstract
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Background: Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide.
Objectives: A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy.
Methods: In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vivo after gene transfer.
Results: The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2.
Conclusions: The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.
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KEYWORD
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Porcine circovirus type 2, recombinant adeno-associated virus 8, capsid protein
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