KMID : 0893420210220020020
|
|
Journal of Veterinary Science 2021 Volume.22 No. 2 p.20 ~ p.20
|
|
Establishment of inflammatory model induced by Pseudorabies virus infection in mice
|
|
Ren Chun-Zhi
Hu Wen-Yue Zhang Jin-Wu Wei Ying-Yi Yu Mei-Ling Hu Ting-Jun
|
|
Abstract
|
|
|
Background: Pseudorabies virus (PRV) infection leads to high mortality in swine. Despite extensive efforts, effective treatments against PRV infection are limited. Furthermore, the inflammatory response induced by PRV strain GXLB-2013 is unclear.
Objectives: Our study aimed to investigate the inflammatory response induced by PRV strain GXLB-2013, establish an inflammation model to elucidate the pathogenesis of PRV infection further, and develop effective drugs against PRV infection.
Methods: Kunming mice were infected intramuscularly with medium, LPS, and different doses of PRV-GXLB-2013. Viral spread and histopathological damage to brain, spleen, and lung were determined at 7 days post-infection (dpi). Immune organ indices, levels of reactive oxygen species (ROS), nitric oxide (NO), and inflammatory cytokines, as well as levels of activity of COX-2 and iNOS were determined at 4, 7, and 14 dpi.
Results: At 105?106 TCID50 PRV produced obviously neurological symptoms and 100% mortality in mice. Viral antigens were detectable in kidney, heart, lung, liver, spleen, and brain. In addition, inflammatory injuries were apparent in brain, spleen, and lung of PRV-infected mice. Moreover, PRV induced increases in immune organ indices, ROS and NO levels, activity of COX-2 and iNOS, and the content of key pro-inflammatory cytokines, including interleukin (IL)-1¥â, IL-6, tumor necrosis factor-¥á, interferon-¥ã and MCP-1. Among the tested doses, 102 TCID50 of PRV produced a significant inflammatory mediator increase.
Conclusions: An inflammatory model induced by PRV infection was established in mice, and 102 TCID50 PRV was considered as the best concentration for the establishment of the model.
|
|
KEYWORD
|
|
Pseudorabies virus, inflammatory, cytokines, mice
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|