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KMID : 0893420210220060066
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Journal of Veterinary Science
2021 Volume.22 No. 6 p.66 ~ p.66
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Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China
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Zhao Ling
Zhang Liang
Shi Xiaona
Duan Xujie
Li Huiping
Liu Shuying
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Abstract
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Background: Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide.
Objectives: In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated.
Methods: Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis.
Results: A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%?86.8% and 67.7%?75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5).
Conclusions: The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.
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KEYWORD
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Maedi/Visna virus, next-generation sequencing, phylogenetic analysis, sheep lung
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