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KMID : 0893420220230010016
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Journal of Veterinary Science
2022 Volume.23 No. 1 p.16 ~ p.16
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Xylazole inhibits NO-cGMP pathway in fetal rat nerve cells
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Wang Xinyu
Wu Yue
Liu Lin
Bai Hui
Zhang Zhiheng
Zhao Mingchao
Ma Tianwen
Song Xiaopeng
Jia Lina
Lv Liangyu
Yu Yue
Xu Xinyu
Chen Hong
Gao Li
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Abstract
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Background: Xylazole (Xyl) is a veterinary anesthetic that is structurally and functionally similar to xylazine. However, the effects of Xyl in vitro remain unknown.
Objectives: This study aimed to investigate the anesthetic mechanism of Xyl using fetal rat nerve cells treated with Xyl.
Methods: Fetal rat nerve cells cultured for seven days were treated with 10, 20, 30, and 40 μg/ mL Xyl for 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, and 120 min. Variations of amino acid neurotransmitters (AANTs), Nitric oxide-Cyclic GMP (NO-cGMP) signaling pathway, and ATPase were evaluated.
Results: Xyl decreased the levels of cGMP and NO in nerve cells. Furthermore, Xyl affected the AANT content and Na+-K+-ATPase and Ca2+-Mg2+-ATPase activity in nerve cells. These findings suggested that Xyl inhibited the NO-cGMP signaling pathway in nerve cells in vitro.
Conclusions: This study provided new evidence that the anesthetic and analgesic effects of Xyl are related to the inhibition of the NO-cGMP signaling pathway.
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KEYWORD
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Xylazole, anesthesia, cyclic GMP, gamma-aminobutyric acid, glutamic acid.
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