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KMID : 0893420220230020018
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Journal of Veterinary Science
2022 Volume.23 No. 2 p.18 ~ p.18
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First molecular detection of canine herpesvirus 1 (CaHV-1) in the Eastern Brazilian Amazon
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Marcela dos Santos Castro
Maridelzira Betania Moraes David
Evonnildo Costa Goncalves
Andrei Santos Siqueira
Rodrigo Rodrigues Virgulino
Delia Cristina Figueira Aguiar
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Abstract
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Background : Canine herpesvirus type 1 (CaHV-1) infects dogs and is associated with neonatal deaths and reproductive, ocular, neurological, and respiratory problems. In Brazil, reports of CaHV-1 have been restricted to the southeast and south regions, particularly in municipalities in the state of Rio Grande do Sul.
Objectives : To assess the presence and variability of CaHV-1 in canine populations in the state of Para, North Brazil.
Methods : Biological samples from 159 dogs from 4 municipalities in the State of Para were evaluated using polymerase chain reaction and phylogenetic analyses, with the target being the viral enzyme, thymidine kinase.
Results : CaHV-1 was detected in 13 dogs (8.2%), with 2 animals being from the municipality of Santa Barbara do Para, 8 from Algodoal Island, 2 from Salinopolis, and one from Capanema. The study sequences revealed 100% identity among themselves and 64% to 100% identity with the other nucleotide sequences from Australia, Brazil, United Kingdom, and United States, including 100% identity with the 2002 isolate from Australia. The 1996 isolate from France was grouped in a branch that was different from the sequence of this study.
Conclusions : This study presents the first molecular detection of CaHV-1 in dogs from the Amazon region in northern Brazil. The nucleotide identity between the strains and cytosine insertion in the sequences isolated in this study suggests at least 2 strains of CaHV-1 circulating in Brazil (Para and BTU-1).
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KEYWORD
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Canine herpesvirus, molecular diagnosis, epidemiology, the Amazon
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