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KMID : 0893420230240010015
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Journal of Veterinary Science
2023 Volume.24 No. 1 p.15 ~ p.15
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Expression of FMD virus-like particles in yeast Hansenula polymorpha and immunogenicity of combine with CpG and aluminum adjuvant
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Jianhui Zhang
Jun Ge
Juyin Li
Jianqiang Li
Yong Zhang
Yinghui Shi
Jiaojiao Sun
Qiongjin Wang
Xiaobo Zhang
Xingxu Zhao
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Abstract
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Background : Inactivated vaccines are limited in preventing foot-and-mouth disease (FMD) due to safety problems. Recombinant virus-like particles (VLPs) are an excellent candidate for a novel vaccine for preventing FMD, given that VLPs have similar immunogenicity as natural viruses and are replication- and infection-incompetent.
Objectives : The 3C protease and P1 polyprotein of type O FMD virus (FDMV) was expressed in yeast Hansenula polymorpha to generate self-resembling VLPs, and the potential of recombinant VLPs as an FMD vaccine was evaluated.
Methods : BALB/c mice were immunized with recombinant purified VLPs using CpG oligodeoxynucleotide and aluminum hydroxide gel as an adjuvant. Cytokines and lymphocytes from serum and spleen were analyzed by enzyme-linked immunosorbent assay, enzyme-linked immunospot assay, and flow cytometry.
Results : The VLPs of FMD were purified successfully from yeast protein with a diameter of approximately 25 nm. The immunization of mice showed that animals produced high levels of FMDV antibodies and a higher level of antibodies for a longer time. In addition, higher levels of interferon-γ and CD4+ T cells were observed in mice immunized with VLPs.
Conclusions : The expression of VLPs of FMD in H. polymorpha provides a novel strategy for the generation of the FMDV vaccine.
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KEYWORD
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Yeast Hansenula polymorpha, food-and-mouth disease, virus-like particles, immunogenicity
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