KMID : 0900220110180020116
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´ëÇѺñ°úÇÐȸÁö 2011 Volume.18 No. 2 p.116 ~ p.121
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Berberine Suppresses Interleukin-1¥â-Induced MUC5AC Gene Expression in Human Airway Epithelial Cells
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Kim Kyung-Rok
Kim Na-Hyun Park Yuna Kim Ji-Hoon Lee Eun-Jung Kim Kyung-Su
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Abstract
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Objectives:The aim of this study was to investigate whether berberine suppresses interleukin (IL)-1¥â-induced MUC5AC gene expression in human airway epithelial cells and, if so, to determine which mitogen-activated protein kinases (MAPKs) are related to MUC5AC gene suppression.
Materials and Methods:MUC5AC mRNA and protein levels were measured using reverse transcription-polymerase chain reaction (PCR), real-time PCR, and western blot analysis in cultured NCI-H292 human airway epithelial cells.
Results:IL-1¥â-induced expressions of MUC5AC mRNA and protein were significantly suppressed in cells pretreated with 25 ¥ìM of berberine. Levels of MAPK proteins were determined by western blot analysis after pretreatment with 25 ¥ìM berberine. Berberine suppressed phosphorylation of extracellular signal-regulated kinase (ERK) and p38 MAPK, but there was no change in the expression of JNK. Suppression of IL-1¥â-induced MUC5AC mRNA was also observed in cells pretreated with ERK- or p38 MAPK-specific inhibitors, suggesting that berberine suppresses IL-1¥â-induced expression of MUC5AC mRNA, which involves the ERK- and p38 MAPK-dependent pathways.
Conclusion:Berberine suppresses IL-1¥â-induced MUC5AC gene expression in human airway epithelial cells via the ERK- and p38 MAPK-dependent pathways; therefore, berberine may be considered as a possible anti-hypersecretory agent for inflammatory airway diseases.
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KEYWORD
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Berberine , Mucin 5AC , Epithelial cells , Mitogen-activated protein kinases
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