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KMID : 0900920030270030187
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Korean journal of Animal Reproduction
2003 Volume.27 No. 3 p.187 ~ p.195
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Study of Efficient Production of Cloned Embryos in Porcine II. Establishment of Conditional Enucleation
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Kang Man-Jong
Moon Seung-Ju
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Abstract
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This study was carried out to improve of enucleation efficiency on porcine recipient oocytes preactivated. In ethanol or Ca^{2+} ionophore, effect of repeating and combinational activation with 6-DMAP or cycloheximide compared with alone activated treatment. Recipient oocytes’s activation by Ca^{2+} ionophore combined with 6-DMAP or cycloheximide were significantly higher than alone treatment(P<0.05). Between repeating and alone treatments were not significantly different. In ethanol, repeating treatment was significantly lower than alone(P<0.05), and combination treatments were not significantly different. On the basis of these results, efficiency of enucleation, electrical fusion and in vitro development compared preactivated with non-preactivated recipient oocytes. Enucleation and fusion rates of preactivated oocytes were improved significantly compared with non-preactivated oocytes(90.7%, 71.8 vs 77.8%, 61.1%; P<0.05). Behind the back, cleavage and in vitro development rates were significantly lower than non-preactivated oocytes(38.7%, 19.3% vs 68.8%, 30.6%; P<0.05).
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KEYWORD
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Enucleation, Recipient oocytes, Preactivation, Combinational activation
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