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KMID : 0921620070370030193
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Journal of Bacteriology and Virology
2007 Volume.37 No. 3 p.193 ~ p.201
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5` and 3` cis-Acting RNA Elements Required for RNA Replication of Porcine Reproductive and Respiratory Syndrome Virus
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Kang Shien-Young
Choi Yu-Jeong
Yun Sang-Im
Song Byung-Hak
Lee Young-Min
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Abstract
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Porcine reproductive and respiratory syndrome virus (PRRSV), a member of the genus Arterivirus in the family Arteriviridae, is the most important viral pathogens in swine industry worldwide. Here, we have investigated 5’ and 3’ cis-acting RNA elements required for PRRSV genome replication. Using the infectious PRRSV cDNA, we have manipulated the genomic RNA to generate mutant genomic RNAs, transfected these mutants into susceptible MARC-145 cells, and examined the competence of RNA replication. We found three genetic factors that were essential for viral replication. First, the cap structure present at the 5’-end of the genome was absolutely required for RNA replication. Secondly, polyadenylation of the genomic RNA at the 3’-end was also essential for RNA replication. Thirdly, approximately 100-nucleotide region just upstream of the N protein-coding region was crucial for genomic RNA replication. Taken together, our findings indicate that replication of PRRSV genomic RNA requires three important cis-acting RNA elements: 5’ cap structure, 3’ poly(A) motif, and an internal sequence of about 100 nucleotides. Further investigation is needed to elucidate the molecular mechanism(s) of how these elements act on PRRSV genome replication.
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KEYWORD
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Porcine reproductive and respiratory syndrome virus, Arterivirus, RNA replication, cis-acting elements
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