KMID : 0921620070370030193
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Journal of Bacteriology and Virology 2007 Volume.37 No. 3 p.193 ~ p.201
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5` and 3` cis-Acting RNA Elements Required for RNA Replication of Porcine Reproductive and Respiratory Syndrome Virus
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Kang Shien-Young
Choi Yu-Jeong Yun Sang-Im Song Byung-Hak Lee Young-Min
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Abstract
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Porcine reproductive and respiratory syndrome virus (PRRSV), a member of the genus Arterivirus in the family Arteriviridae, is the most important viral pathogens in swine industry worldwide. Here, we have investigated 5¡¯ and 3¡¯ cis-acting RNA elements required for PRRSV genome replication. Using the infectious PRRSV cDNA, we have manipulated the genomic RNA to generate mutant genomic RNAs, transfected these mutants into susceptible MARC-145 cells, and examined the competence of RNA replication. We found three genetic factors that were essential for viral replication. First, the cap structure present at the 5¡¯-end of the genome was absolutely required for RNA replication. Secondly, polyadenylation of the genomic RNA at the 3¡¯-end was also essential for RNA replication. Thirdly, approximately 100-nucleotide region just upstream of the N protein-coding region was crucial for genomic RNA replication. Taken together, our findings indicate that replication of PRRSV genomic RNA requires three important cis-acting RNA elements: 5¡¯ cap structure, 3¡¯ poly(A) motif, and an internal sequence of about 100 nucleotides. Further investigation is needed to elucidate the molecular mechanism(s) of how these elements act on PRRSV genome replication.
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KEYWORD
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Porcine reproductive and respiratory syndrome virus, Arterivirus, RNA replication, cis-acting elements
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