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KMID : 0923620110110010042
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Immune Network
2011 Volume.11 No. 1 p.42 ~ p.49
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Anti-inflammatory and Anti-oxidative Effects of Korean Red Ginseng Extract in Human Keratinocytes
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Hong Chang-Eui
Lyu Su-Yun
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Abstract
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Background: In this study, we have investigated the effect of Korean red ginseng (KRG) extracts on the production of TNF-? and IL-8 in human keratinocytes. Also, to examine the antioxidative effect of red ginseng extracts, free radical scavenging activity and superoxide dismutase (SOD) activity in human dermal fibroblasts was measured.
Methods: To investigate the effect of KRG in atopic dermatitis, we measured the level of TNF-? and IL-8 secretion in LPS-stimulated human keratinocytes after the treatment of KRG extracts using enzyme-linked immunosorbent assay. Anti-oxidative activity was investigated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and SOD activity.
Results: The stimulation of human keratinocytes with KRG extracts shifted the LPS-induced cytokine secretion toward a more immunosuppressive response. KRG dose-dependently decreased TNF-? and IL-8 production in HaCaT cells and a significant inhibition of TNF-? was shown when cells were treated with 500 and 1,000?g/ml of KRG extracts. Additionally, KRG extracts showed DPPH radical scavenging and SOD activity in a dose-dependent manner. Particularly, SOD activities of concentrations higher than 60?g/ml of KRG extracts were significantly different in human dermal fibroblast cells.
Conclusion: Based on this study, KRG extracts may be a useful immunosuppressive agent in the treatment of atopic dermatitis.
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KEYWORD
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IL-8, TNF-?, Anti-oxidation, Korean red ginseng, Atopic dermatitis
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