KMID : 0923620130130040123
|
|
Immune Network 2013 Volume.13 No. 4 p.123 ~ p.132
|
|
Metformin Down-regulates TNF-¥á Secretion via Suppression of Scavenger Receptors in Macrophages
|
|
Hyun Bo-Bae
Shin Seul-Mee Lee Ae-Ri Lee Sung-Won Song Young-Cheon Ha Nam-Joo Cho Kyung-Hea Kim Kyung-Jae
|
|
Abstract
|
|
|
Obesity is consistently increasing in prevalence and can trigger insulin resistance and type 2 diabetes. Many lines of evidence have shown that macrophages play a major role in inflammation associated with obesity. This study was conducted to determine metformin, a widely prescribed drug for type 2 diabetes, would regulate inflammation through down- regulation of scavenger receptors in macrophages from obesity-induced type 2 diabetes. RAW 264.7 cells and peritoneal macrophages were stimulated with LPS to induce inflammation, and C57BL/6N mice were fed a high-fat diet to generate obesity-induced type 2 diabetes mice. Metformin reduced the production of NO, PGE2 and pro-inflammatory cytokines (IL-1¥â, IL-6 and TNF-¥á) through down-regulation of NF-¥êB translocation in macrophages in a dose-dependent manner. On the other hand, the protein expressions of anti-inflammatory cytokines, IL-4 and IL-10, were enhanced or maintained by metformin. Also, metformin suppressed secretion of TNF-¥áand reduced the protein and mRNA expression of TNF-¥á in obese mice as well as in macrophages. The expression of scavenger receptors, CD36 and SR-A, were attenuated by metformin in macrophages and obese mice. These results suggest that metformin may attenuate inflammatory responses by suppressing the production of TNF-¥á and the expressions of scavenger receptors.
|
|
KEYWORD
|
|
Metformin, Obesity-induced inflammation, TNF-¥á, Scavenger receptors
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|