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KMID : 1007520180270020591
Food Science and Biotechnology
2018 Volume.27 No. 2 p.591 ~ p.598
Separation and identification of bromelain-generated antibacterial peptides from Actinopyga lecanora
Ghanbari Raheleh

Ebrahimpour Afshin
Abstract
Actinopyga lecanora, as a rich protein source was hydrolysed to generate antibacterial bioactive peptides using different proteolytic enzymes. Bromelain hydrolysate, after 1 h hydrolysis, exhibited the highestantibacterial activities against Pseudomonas aeruginosa, Pseudomonas sp., Escherichia coli and Staphylococcus aureus. Two dimensional fractionation strategies, using a semi-preparative RP-HPLC and an isoelectric-focusing electrophoresis, were applied for peptide profiling. Furthermore, UPLC-QTOF-MS was used for peptides identification; 12 peptide sequences were successfully identified. The antibacterial activity of purified peptides from A. lecanora on P. aeruginosa, Pseudomonas sp., E. coli and S. aureus was investigated. These identified peptides exhibited growth inhibition against P. aeruginosa, Pseudomonas sp., E. coli and S. aureus with values ranging from 18.80 to 75.30%. These results revealed that the A. lecanora would be used as an economical protein source for the production of high value antibacterial bioactive peptides.
KEYWORD
Actinopyga lecanora, Bioactive peptides, Antibacterial activity, Hydrolysate, Proteolytic enzyme
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