|
KMID : 1007520210300131709
|
|
Food Science and Biotechnology
2021 Volume.30 No. 13 p.1709 ~ p.1719
|
|
|
Optimization the extraction of anthocyanins from blueberry residue by dual-aqueous phase method and cell damage protection study
|
|
Gao Yuan
Ji Yubin
Wang Fuling
Li Wenlan
Zhang Xiaomeng
Niu Zhihui
Wang Zhuo
|
|
|
Abstract
|
|
|
|
Blueberry residue is usually discarded as waste, but has a high anthocyanins content. The extraction method of anthocyanins from blueberry residue with ultrasonic assisted dual-aqueous phase system was optimized. In terms of the principle of central group and design (CCD) experimental design, three-factor and five-level response surface analysis was adopted to optimize the extraction conditions with the extraction rate of anthocyanins. The optimum extraction rate of anthocyanin was 12.372?±?0.078 mg/g. Anthocyanin extract could protect the pBR322 DNA oxidative damage induced by Fenton reagent, increase the superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px) enzyme activities, and decrease the H2O2-induced cell apoptosis of human normal liver cell (LO2 cell). The study indicates that the extraction rate of anthocyanin was increased by optimized ultrasonic assisted dual-aqueous phase system. The anthocyanin extract could protect DNA and LO2 cell from oxidative damage.
|
|
|
KEYWORD
|
|
|
Blueberry residue, Anthocyanin, Dual-aqueous phase, SOD, GSH-Px
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|