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KMID : 1012320030060010012
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Nutritional Sciences
2003 Volume.6 No. 1 p.12 ~ p.19
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A selective Assay To Measure Antioxidant Capacity in Both The Aqueous and Lipid Compartments of Plasma
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Giancarlo Aldini
Yeum Kyung-Jin
Robert M. Russel
Norman I. Krinsky
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Abstract
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The measurement of the total antioxidant capacity (TAC) of human plasma has been widely applied in nutritional science, for example to evaluate the antioxidant contribution of dietary components and to study, although indirectly, the bioavailability of dietary antioxidants. Several methods have been proposed for the measurement of TAC, most of them based on the ability of plasma to withstand the oxidative damage induced by aqueous radicals. Although plasma contains both hydrophilic and lipophilic antioxidants that interact through extensive cross-talk in most of the methods employed for the TAC measurement, the hydrophilic antioxidants such as ascorbic acid, uric acid, and protein thiols mainly contribute to the total antioxidant plasma capacity (almost 70%) while lipophilic antioxidants embedded in the lipoproteins (carotenoids, a-tocopherol, ubiquino1-10) participate only in a negligible amount (less than 5%). The present paper reviews the analytical methods used to assess the TAC and in particular focuses on new approaches that are capable of distinguishing the antioxidant capacity of both the aqueous and lipid compartments of plasma. The general principle of the method as well as some in vitro and ex vivo applications will be discussed within the text.
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KEYWORD
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total antioxidant capacity, aqueous and lipid plasma compartments
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