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KMID : 1012320040070010008
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Nutritional Sciences
2004 Volume.7 No. 1 p.8 ~ p.16
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Gene Expression Profile in the Liver Tissue of High Fat Diet-Induced Obese Mice
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Cha Min-Ho
Kang Bong-Joo
Kim Kyung-Seon
Sim Woong-Seop
Oh Hyun-Hee
Yoon Yoo-Sik
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Abstract
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The purpose of this study was to investigate the gene profiles that were up- or down-regulated in the livers of high-fat diet-induced obese mice and db_/db_mice with deficient leptin receptor. C57/BL6 normal mice and db_/db_mice, respectively, were divided into two groups and fed a standard or high-fat diet for four weeks. Liver weight was unchanged in the normal mice but the high-fat diet led to a 10% weight increase in the db_/db_mice. Adipose tissue mass increased by about 88% in the normal mice that were fed a high-fat diet and by about 17% in the db_/db_mice on the high-fat diet. In terms of serum lipids, total cholesterol significantly increased in mice on the high-fat diet. Microarray analysis was carried out using total RNA isolated from the livers of standard or high-fat diet-fed mice of the normal and db_/db_strains. The change of gene expression was confirmed by RT-PCR. About 1.6% and 6.8% of total genes, respectively, showed different expression patterns in the normal mice fed the high-fat diet and db_/db_mice. As a result of microarray, many genes involved in metabolism and signal pathways were shown to have different expression patterns. Expression of Mgst3 gene increased in the livers of normal and db_/db_mice that were fed a high-fat diet. Wnt7b and Ptk9l were down-regulated in the livers of the normal mice and db_/db_mice that were fed a high-fat diet. In conclusion, a high-fat diet induced obesity and affected gene expression involved in metabolism and signal pathway.
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KEYWORD
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High fat diet, Wnt7b, Ptk91, Liver, Microarray, db_/db_mouse
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