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KMID : 1012320060090010003
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Nutritional Sciences
2006 Volume.9 No. 1 p.3 ~ p.8
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Inhibitory Effects of (-) Epigallocatechin Gallate and Quercetin on High Glucose-induced Endothelial Cytotoxicity
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Choi Yean-Jung
Kwon Hyang-Mi
Choi Jung-Suk
Bae Ji-Young
Kang Sang-Wook
Lee Sang-Soo
Lee Yong-Jin
Kang Young-Hee
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Abstract
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Functional damage to microvascular endothelial cells by hyperglycemia is thought to be one of the critical risk factor.; in the impaired wound healing seen with diabetes mellitus. It is also thought that oxidative stress plays a significant role in this endothelial cell dysfunction. The present study examined the differential effects of flavonoids on endothelial cell dysfunction under high glucose conditions. Human endothelial cells exposed to 30 mmol/L glucose for 7 d were pre-treated with various flavonoids and pulse-treated with 0.2 mmol/L H2O2 30 min. High glucose markedly decreased cell viability with elevated oxidant generation and nuclear condensation. H2O2 insult exacerbated endothelial cytotoxicity due to chronic exposure to high glucose. (-)Epigallocatechin gallate and quercetin improved glucose-induced cell damage with the disappearnnce of apoptotic bodies, whereas apigenin intensified the glucose cytotoxicity. In addition, cell viability data revealed that these flavonoids of (-)epigallocatechin gallate and quercetin substantially attenuated both high glucose- and H2O2- induced dual endothelial damage. These results suggest that (-)epigallocatechin gallate and quercetin may be beneficial agents for improving endothelial cell dysfunction induced by high glucose and may prevent or reduce the development of diabetic vascular complications.
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KEYWORD
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Diabetes, Endothelial cells, Flavonoids, High glucose, Oxidative stress
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