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KMID : 1012320060090040317
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Nutritional Sciences
2006 Volume.9 No. 4 p.317 ~ p.322
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An Evaluation of Changes in the Allergenicity of Kochujang upon Preparation Using Aloe Extract
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Son Bo-Kyung
Huh Yoon-Ee
Kim Jung-Yun
Noh Geon-Woong
Lee Sang-Sun
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Abstract
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Soybeans are well-known as allergenic foods. Koreans consume large amounts of soybean foods, such as kochujang, which have gone through the fermentation process. To lower the allergenicity of these foods, we prepared hypo allergenic kochujang with aloe extract (AK). A sensory evaluation was conducted along with a clinical evaluation that used a double-blind, placebo-controlled food challenge (DBPCFC) test These tests were designed to evaluate the acceptability of the fermented foods. In comparison to normal kochujang (NK), AK elicited a higher sensory test score, and the rate of positive reactions in atopic dermatitis patients during the DBPCFC test was reduced. Methods of protein extraction, protein quantitation with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), and protein identification using two-dimensional (2D) gel electrophoresis were performed for both NK and AK to compare the functional factors. We found a reduction in the levels of high molecular proteins even though the bands of the proteins had not entirely disappeared, indicating that the boiling and fermentation process changed the soybean protein patterns. The rate of the reduction of high molecular proteins was more effective in the AK. In conclusion, AK can be recognized as a food with hypoallergenic effect.
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KEYWORD
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Kochujang, Allergenicity, Atopic dermatitis, Aloe, DBPCFC, SDS-PAGE, 2D electrophoresis
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