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KMID : 1023519980210020107
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Korean Journal of Veterinary Service
1998 Volume.21 No. 2 p.107 ~ p.115
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Studies on enzyme-linked immunosorbent assay(ELISA) for detection of antibody to Brucella abortus
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Shim Hang-Sub
Kook Jung-Hee
Jung Bong-Su
Ko Tae-Oh
Cho Jung-Hyun
Park Yu-Soon
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Abstract
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In order to establish a rapid, sensitive and specific diagnostic method for detection of antibody to Brucella abortus, a enzyme-linked immunosorbent assay(ELISA) was adapted. The diagnostic efficacy of the established ELISA was compared with that of the standard tube agglutination test for B abortus. 1. It was found that the optimal concentration of antigen for this ELISA was 5/ml, the optimal dilution of conjugate was 1 : 2000, and the optimal dilution of serum was 1 : 200, respectively. 2. Cut off value in this ELISA was 1,102 that was determined by mean absorbance(at 492nm) of tube agglutination test negative serum added with the triple value of the standared devation. 3. The relationship between the tube agglutination test and ELISA was showen high corresponding rate with sensitivity(96.3%) and specificity(98.1%). 4. The efficacy of the ELISA for detection of B abortus antibody was compared with tube agglutination test In brucellosis outbreak farm. The sensivity of ELSIA was higher than tube agglutination test.
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KEYWORD
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Brucella abortus, ELISA, Tube agglutination test
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