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KMID : 1023520010240010083
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Korean Journal of Veterinary Service
2001 Volume.24 No. 1 p.83 ~ p.88
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Detection of equine herpesvirus type-1 in naturally aborted equine fetuses in Jeiu by polymerase chain reaction
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Moon Hyuk
Kang Wan-Cheul
Kim Eun-Joo
Kim Jin-Hoe
Ko Hyun-Joo
Yang Jae-Hyuk
Son Won-Geun
Lee Du-Sik
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Abstract
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It is impotent to identify the causative agents of abortion in equine for minimizing the loss of breeding costs in equine Industry. Recently, the abortion has often occurred in equine herds and thus the purpose of the study was aimed at the identification of equine herpesvirus-1, one of the frequent pathogens to abortion, using polymerase chain reaction. Six fetuses to be aborted at nine to ten months in pregnancy reared in six herds were used in the study. Two primers in the PCR were made from glycoprotein B gene of EHV-1. The primers specific for EHV-1 amplified 1880 bp of PCR products from DNA extracts from thorax fluids, livers, lungs, and spleens of four in six aborted fetuses. Consequently, PCR could be applied to diagnose the abortion of EHV-1 and also confirmed to play a major role of the viral pathogen associated with equine abortion in Jeju island.
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KEYWORD
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Equine, EHV-1, Abortion, PCR, Equine rhinopneumonitis
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