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KMID : 1023520020250020135
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Korean Journal of Veterinary Service
2002 Volume.25 No. 2 p.135 ~ p.140
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Detection of Mycobacterium bovis in cattle by PCR
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Kong Shin-Koog
Lee Gun-Taek
Lim Jong-Mook
Yang Seung-Min
Lee Jho-An-Na
Moon Sun-Hwa
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Abstract
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The purpose of this study was to detect Mycobacterium bovis in cattle(serum, milk, lung, lymph node) by PCR. Nineteen samples from 7 skin test-positive cattle were analyzed. The amplified band of IS6110 by PCR was detected from 2 samples in lung and Iymph node. But the sensitivities of the present methods for detecting M bovis in milk and serum are deficient. Because the PCR sensitivity has been shown to be hindered by the method used to isolate the nucleic acid target. PCR-based methods have the potential to be faster, more accurate, and the most efficient means of detecting M bovis. The detection of M bovis by PCR will contribute to the more efficient detection and control of tuberculosis.
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KEYWORD
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Mycobacterium bovis, PCR
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