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KMID : 1023520020250030295
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Korean Journal of Veterinary Service
2002 Volume.25 No. 3 p.295 ~ p.297
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Immunohistochemical detection of infectious hematopoietic necrosis virus antigens in cell cultures
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Moon Oun-Kyong
Lee Min-Kwon
Jin Young-Bae
Kim Soon-Bok
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Abstract
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This experiment was done to set up the immunohistochemical detection method for infectious hematopoietic necrosis virus(IHNV) antigens in the monolayers of CHSE-214 cell cultures inoculated with IHNV. Specific identification of IHNV antigens was detected in the cytoplasms of infected cells by the use of monoclonal antibodies to glycoproteins. The specific positive signal was observed as a distinct red color. The result showed that streptavidin alkaline phosphatase immunohistochemistry specifically identified IHNV antigens in infected cultured cells.
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KEYWORD
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Immunohistochemistry, Infectious hematopoietic necrosis virus, Cell culture
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