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KMID : 1023520020250040333
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Korean Journal of Veterinary Service
2002 Volume.25 No. 4 p.333 ~ p.346
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Mapping of the equine herpesvirus type 1 immediate-early protein interaction domain within the general transcription factor human TFIIB
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Jang Hyung-Kwan
Cho Jeong-Gon
Song Hee-Jong
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Abstract
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We previously reported that the equine herpesvirus type 1(EHV-1) immediate-early protein(IE protein) physically interacts with the general transcription factor human TFIIB(Jang et al, J Virol 75：10219-10230, 2001). The interaction between the IE protein and TFIIB is necessary for the IE protein to efficiently transactivate the early TK and late IR5 EHV-1 promoters. A panel of deletion and truncation mutants of the TFIIB gene was constructed and employed in protein-binding assays to map the IE protein-binding domain within TFIIB. Evidence is presented that the first direct repeat of TFIIB interacts specifically with the EHV-1 IE protein.
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KEYWORD
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Equine herpesvirus type 1, EHV-1, IE protein, TFIIB
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