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KMID : 1023520050280020121
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Korean Journal of Veterinary Service
2005 Volume.28 No. 2 p.121 ~ p.146
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Studies of the egg drop laying diseases from the mass zone layer
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Lee Jeoung-Won
Eum Sung-Shim
Park In-Gyu
Bae Joung-Jun
Joung Dong-Suk
Song Hee-Jong
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Abstract
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Newcastle disease (ND), infectious bronchitis (IB), low pathogenic avian Influenza (LPAI) and fowl typhoid (FT) have been known as egg drop laying diseases because of the serious layer damage from mass zone layer. In this study, such egg drop laying diseases were investigated. To access this study, we peformed to evaluate antibody titers in serum and isolated bacteria and virus from organs and feces on May, July and September in 2003. The distribution of ND from January to May, IB and LPAI from October to February of the next year, and FT from March to September were inspected by the question survey in 21 farms. ND revealed to be positive rates of 490 to 474 in May, 510 to 506 in July and 510 to 510 in September with hemagglutination inhibition (HI) test. The mean antibody titers were 10.2, 9.9 and 10.2, respectively. With regard to IB, 484 out of 490 samples in May, 508 of 510 in July and 509 of 510 in September showed positive results and the mean antibody titers were gradually increased with 8.2, 9.0 and 9.4, respectively. According to HI test of LPAI, the positive results were shown in 442 of 480 , 394 of 494 and 402 of 483 in May, July and September, respectively The mean antibody titers were decreased with 4.6, 4.3 and 4.0. The distribution of LPAI also elicited the positive rates of 480 to 475 in May, 494 to 485 in July, 483 to 472 in September as determined by ELISA and the mean S/P ratio were 2.319, 2.557 and 2.380, respectively. Compared ELISA results with HI test of LPAI the positive results were 480 to 422 . Therefore, the positive rate determined by ELISA was higher than that of HI test with 6.9, 18.4 and , respectively. When performed RT-PCR for ND using organ and feces samples, the pathotypes were detected in September but there is no samples showing positive band for LPAI. In attempt to isolate Salmonella gallinarum, bacteria were obtained from 4 cases (12.5%) in May, 9 (23.6%) in July, 5 (17.8%) in September. Thus the highest rate for isolation revealed to be shown in July When evaluated the antimicrobial susceptibility to 18 isolated strains of 5. gallinarum, bacteria were sensitive to trimethoprim/sulfamethox and amoxacillin/clavulanic acid , cephalothin , but resistant to penicillin , streptomycin , erythromycin and tetracycline . According to HI test of ND and LPAI using captured 164 wild Korean tree sparrows (Passer nontanus), the positive rates were , and the mean HI titers were 5.32 and 4.02, respectively. 71 in captured sparrows also showed more than 4 titers for HI test to ND and LPAI, respectively However, the attempt for isolation of viruses failed in all samples.
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KEYWORD
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Egg drop laying diseases, Mass zone layer, Newcastle disease, Infectious bronchitis, Avian influenza, Fowl typhoid, HI test, ELISA, Antimicrobial susceptibility
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