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KMID : 1023520060290010001
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Korean Journal of Veterinary Service
2006 Volume.29 No. 1 p.1 ~ p.8
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Application of PCR for diagnosis of porcine circovirus type 2
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Park Hyo-Sun
Lee Hyo-Sang
Na Ki-Bok
Lee Kwan-Bok
Kang Su-Jung
Moon Sun-Hwa
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Abstract
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Porcine circovirus (PCV) is a small, nonenveloped virus that contains a single-stranded circular DNA genome of about 1.76 kb and belongs to the family circoviridae. The PCV-2 has been incriminated as the cause of post-weaning multisystemic wasting syndrome (PMWS) , an emerging disease in pigs. In the present study, a PCR assay was applied to detect PCV-2 in tissue samples. The presence of PCV-2 antigen in the porcine tissues was confirmed by indirect immunofluorescence (IIF) with PCV-2 specific monoclonal antibodies. And then DNA extracted from PCV-2 positive tissues was used as a template. One oligonucleotide primer suitable for PCR was selected from a published PCV-2 sequence (Genbank). Amplified PCR product was detected the same fragment lengths of 416 bp as a control. Based on these results, it was suggested that the PCR is a simple and sensitive method for support diagnostic purposes.
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KEYWORD
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Porcine circovirus type-2, Post-weaning multisystemic wasting syndrome, PCR
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