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KMID : 1023520060290020097
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Korean Journal of Veterinary Service
2006 Volume.29 No. 2 p.97 ~ p.102
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Isolation and characterization of Clostridium Perfringens on bovine and porcine carcass
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Chae Hee-Sun
Kim Yeon-Ha
Kim Ju-Young
Kim Jong-Hwa
Kim Kyu-Hiun
Choi Tae-Suk
Sin Bang-Woo
Lee Duck-Joo
Lee Jung-Hark
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Abstract
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A total of 1,848 samples was taken from bovine and porcine from January 2003 to November 2005. They were examined for the presence of Clostridiuml perfringens. The properties of the isolates were characterized for Gram staining, biochemical features and enterotoxin production. Forty-one strains (2.2%) of C perfringens were isolated from the 1,848 bovine and porcine carcass using selective media. 30 (3.2%) C perfringens were isolated from the 925 of bovine cacasses, and 11(1.2%) were isolated from the 923 of porcine cacasses. In TSC agar, all isolates showed lecithinase activity. The isolation rate was higher in spring and summer than in autumn and winter. Among 41 isolates, only 1 isolate detected the cpe gene in PCR. The PCR amplified band was observed 233 bp.
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KEYWORD
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Clostridiuml perfringens, enterotoxin, PCR, cpe gene
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