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KMID : 1023520070300010001
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Korean Journal of Veterinary Service
2007 Volume.30 No. 1 p.1 ~ p.12
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Simultaneous determination of aminoglycoside antibiotics by HPLC/MS
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Roh Young-Sun
Baek Gui-Jung
Kim Seung-Yong
Choi Eun-Young
Seo Heyng-Seok
Hur Boo-Hong
Joung Dong-Suk
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Abstract
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A liquid chromatographic method has been developed for the analysis of aminoglycoside antibiotics (AMGs) using Heptafluorobutyric acid (HFBA) as a ion-pairing reagent. AMGs (amikacin, apramycin, dihydrostreptomycin, gentamicin, hygrosin B, kanamycin, neomycin, spectinomycin and tobramycin) were formed by reaction with HFBA as ion-pairing reagent. HFBA was attached to corresponding amino group of AMGs. These AMGs compounds were separated and detected by electrospray ionization mass spectrometry (ESI-MS). The experimental conditions for separation of AMGs were optimized and validated. A simple liquid chromatographic method for the determination of AMGs was demonstrated.
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KEYWORD
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Aminoglycoside antibiotics (AMGs), Gentamicin, Dihydrostreptomycin, HFBA, ESI-MS
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